In addition, our functional enrichment analysis of the fourth, most differentiated set, identified genes associated with congenital heart diseases (Gata4/6, Hand1/2, Tbx20, Nrp1, and Acta2) such as tetralogy of Fallot, double outlet right ventricle and ventricular septal defects in the most differentiated cells (Supplementary Data 6), which supports the importance of the genetic program of CNCCs in OFT formation and disease. Here, NRP1 is linked to Tetralogy of Fallot.