Our findings identify a specific cluster in the PCA of the 1H‐NMR lipid spectra in eNOS−/− mice during NAFLD progression compared with WT mice that could provide new hypotheses for the metabolic changes in the progression of this disease, and highlights the potential of 1H‐MRS as a noninvasive tool for diagnosis and follow‐up of patients, as other studies have suggested.73, 74. This evidence concerns the gene NOS3 and metabolic dysfunction-associated steatotic liver disease.