More interestingly, by analyzing single-nucleus RNA-seq data, we found that in CHMP5, one of the 15 FIRGs, is dysregulated in ExL2_L3 and ExL3_L5, indicating that in ALS patient, excitatory neurons located in motor cortex layer 2_3 and layer 3_5 have higher CHMP5 expression and may be more vulnerable to ferroptosis and iron metabolism dysfunction. The gene discussed is CHMP5; the disease is amyotrophic lateral sclerosis.