Thereafter, we studied the expression analysis of the 15 hub FIRGs in cell subpopulations in ALS and PN, and found that CHMP5, one of the 15 hub FIRGs, was expressed significantly higher in excitatory neuron populations of ExL2_L3 and ExL3_L5 in ALS than PN (Figure 6D). Here, CHMP5 is linked to amyotrophic lateral sclerosis.