Interestingly, double Pak1/Pak3-knockout mice display hyperactivity, anxiety, learning and memory deficits, secondary microcephaly, reduced synaptic density but increased size of individual synapses and increased basal synaptic transmission, disrupted LTP and LTD, decreased cofilin regulation and reduced CREB activity (Huang et al., 2011). This evidence concerns the gene CREB1 and Anxiety.