To further investigate whether ASIC3 also play a role in MC903-induced atopic dermatitis, we established previous AD mouse model, and blocked ASIC3 by intradermal injection of ASIC3 blocker amiloride (30 nM) at the affected neck skin, the result showed that blocking ASIC3 significantly reduced the scratching behavior of AD mice (t6 = 4.978, p = 0.0025, Figure 6G). This evidence concerns the gene ASIC3 and atopic eczema.