As presented in Table 1, no significant association was observed between the SIX1 expression levels and gender, age, location, tumor differentiation, tumor-node-metastasis (TNM) stage, T stage, N stage, or major vascular invasion, but the SIX1 expression levels correlated positively with nerve infiltration and serum CA199 values (P = 0.046 and P = 0.029, respectively). This evidence concerns the gene SIX1 and neoplasm.