Gating overlay of lymphoid cell subsets in t-sne dimension 1 and 2 indicated lower density of CD4 and CD45 clusters in the spleen (Supplementary Figures 4E, F) at 30 days post-infection, while the density of clusters remained identical for WT and Duox1 KO at 1 day post-infection (Supplementary Figures 4B, C). Here, CD4 is linked to infection.