PDHA1 and central nervous system cancer: More patients with grade 4 glioma were classified as subtype B, whereas more patients with grade 2 glioma were classified as subtype A. The heatmap also showed that most of cuproptosis-related genes were highly expressed in subtype B, while LIAS, ATP7B, PDHA1, and CDKN2A were expressed higher in subtype A. Also subtype B had more patients aged >65.