ESR1 and prostate cancer: Our group has shown the presence of ERα and ERβ by Western blot and immunofluorescence analyses in androgen-independent prostate cancer cells PC-3 and DU-145 and in human post pubertal prostate epithelial cells PNT1A, using different antibodies for ERα and ERβ (67, 68), previously validated (64) and also positive control cells for these receptors (67, 69).