We further investigated whether CLDN6 inhibited the migration and invasion of breast cancer cells through autophagy, we used 3-Methyladenine (3-MA) to inhibit the formation of autophagosome in the early stage of autophagy, and CQ to inhibit the fusion of autophagosome and lysosomes in the late stage of autophagy, respectively (Fig. 1C). The gene discussed is CLDN6; the disease is breast cancer.