CLDN6 and breast carcinoma: We further investigated whether CLDN6 inhibited the migration and invasion of breast cancer cells through autophagy, we used 3-Methyladenine (3-MA) to inhibit the formation of autophagosome in the early stage of autophagy, and CQ to inhibit the fusion of autophagosome and lysosomes in the late stage of autophagy, respectively (Fig. 1C).