APC and colorectal carcinoma: WES showed that APC was the most frequently mutated gene in the WCH cohort, with a mutation frequency of up to 50% (Fig. 1A and supplemental Table S2), consistent with the findings of a total of 1562 CRC patients from six other public databases (Fig. 1B and supplemental Table S1) (20, 35, 36, 37, 38, 39, 40, 41, 42).