As previously reported for PTEN-null melanomas31, 4/5 PTEN-null melanomas showed low immune cell infiltration in the matched tumor dissociates (Fig. 3A and Supplementary Fig. S6), and all PTEN-null melanomas displayed innate resistance to immune checkpoint inhibitors (Table 1; Fisher’s exact test p < 0.05), Consistent with previous reports (reviewed in ref. 36), PTEN-null melanoma cells within tumor dissociates did not consistently display elevated PD-L1 expression, relative to TILs (Supplementary Fig. S6). This evidence concerns the gene CD274 and melanoma.