The cell lines used during the setting are: the FRLT5 cell line (a thyroid normal cell line used as positive control), stimulated and unstimulated with TSH [31], and the BCPAP cell line (a cancer cell line used as the negative control), treated and untreated with SAHA and VPA, two histone deacetylase inhibitors which are known to stimulate the transcription of NIS gene [32]. The gene discussed is SLC5A5; the disease is cancer.