ITGB4 and neoplasm: At the level of the total human DNA per tissue DNA template (Alu-PCR), metastatic cells were mainly present in the lungs of shControl/WT and shControl/KO mice, but not in the lungs of mice from the two shITGB4 groups (Additional file 1: Fig. S1), indicating that ITGB4 is required for the formation of disseminated tumor deposits after i.v. administration.