The possible effect of swertiamarin on protein expression of candidate insulin signalling intermediates viz insulin receptor (INSR-β), phospho insulin receptor substrate [pIRS(ser307)], phosphatidylinositol 3-kinase (PI(3)K), phospho protein kinase B (pAkt), protein kinase C (PKC-ζ), extracellular regulatory kinase (ERK1/2), phospho P38 mitogen activated protein kinase (pP38MAPK) and Peroxisome proliferator-activated receptor gamma (PPAR-γ) in PCOS-IR and NIR hLGCs were analyzed by western blot. The gene discussed is INSR; the disease is polycystic ovary syndrome.