In summary, our findings unveil new roles for the formin DIAPH1 in the regulation of cholesterol and triglyceride metabolism in a manner independent of direct transcriptional regulation of Srebf1, Srebf2, and Mxlipl. This work presents a new lens into DIAPH1 functions in the regulation of hepatic lipid metabolism, actin organization, nuclear translocation of SREBP1 and their collective impact on atherosclerosis. This evidence concerns the gene DIAPH1 and atherosclerosis.