To further investigate the regulatory role of PtpA in ferroptosis during Mtb infection, we deleted the gene encoding ptpA in Mtb H37Rv (Mtb ΔptpA) and complemented Mtb ΔptpA with WT ptpA (Mtb ΔptpA:ptpA) for infection of U937 cells. Here, PTPA is linked to infection.