TNF and infection: Flow cytometry (Fig 4E) and RT-qPCR (Fig 4F and 4G) showed that the levels of effector molecules in hepatic NK cells, such as IFN-γ, Prf1, Gzmb, IL-10, and TNF-α, were higher in Tigit-/- mice than in WT mice at 4 and 6 weeks post infection.