Using this dataset, we first found EM transition-associated alternative splicing events and RNA-binding proteins in breast cancer, and studied the dynamic regulatory relationships between these alternative splicing events and RNA-binding proteins; then we revealed the dynamic regulatory relationships between the alternative splicing events of the CD44 gene and RNA-binding proteins (heterogeneous nuclear ribonucleoprotein M, hnRNPM; epithelial splicing regulatory protein 1, ESRP1; and epithelial splicing regulatory protein 2, ESRP2) during the EM transition process. The gene discussed is ESRP1; the disease is breast cancer.