FACS analysis showed ILC1lc activation when these were co-cultured with ‘stressed,’ autologous HFs (600 cells/HF), as evidenced by significantly increased intracellular IFN-γ expression by ILC1lc (Bernink et al., 2017) (93 ± 11%) compared to the positive control (CD8+/NKG2D+, 75 ± 9%, p<0.05) (Figure 6C) and the negative controls ILC2s, 11 ± 1%, p<0.05; ILC3s, 28 ± 5%, p<0.05; PBMCs/PHA, 2 ± 1.3%, p<0.001 (Figure 6C). This evidence concerns the gene IFNG and Hand-foot syndrome.