In addition, a variety of factors also affect hepatoma by regulating endogenous H2S. CSE is highly expressed in hepatoma cell lines HepG2 and PLC/PRF/5, but low in Hep3B, and its silencing shows an inhibitory effect in HepG2 and PLC/PRF/5, while the effect on Hep3B was not obvious. The gene discussed is CTH; the disease is hepatocellular carcinoma.