Since ZO-1 and several claudin proteins have been reported to be degraded through autophagy-lysosome pathway (Liu et al., 2016; Zhang et al., 2018; Ganapathy et al., 2022), and WT1 was involved in the Akt-JNK pathway dependent autophagy in human osteosarcoma cells (Mo et al., 2016), we speculate that WT1 may regulate the expression of tight junction proteins (ZO-1 and Claudin 11) through autophagy-lysosome pathway, which needs further investigation. This evidence concerns the gene AKT1 and osteosarcoma.