Although inflammation is important for cancer proliferation and metastasis33, multiple studies confirmed the anti-inflammatory role of melatonin treatment in various in vitro cancer models treated with 1 mM melatonin for 24 h via down-regulation of individual inflammatory mediators, such as NF-kB34, IL-1α, IL-1β, TNF-α, COX-2, IL-8 and MCP-19, the present study is the first one which indicates the anti-inflammatory effect of 1 mM melatonin through targeting forty inflammatory mediated factors by using Human inflammation antibody array membrane. The gene discussed is CXCL8; the disease is cancer.