GSEA showed that THCA up, martens tretinoin response up, nuytten NPP1 targets dn, and other GSEA pathways were significantly enriched, and the THCA dn, thyroid carcinoma anaplastic dn, and Nikolsky breast cancer 5p15 amplicon pathways were significantly downregulated in the tumor tissue of patients with THCA (Fig. 4A). This evidence concerns the gene ENPP1 and thyroid gland carcinoma.