Notably, among activator E2Fs, E2F3 appeared to induce ACSL4 expression to the greatest degree in RB-knockdown PC3 cells (Figure 2D) and displayed a stronger correlation with ACSL4 in human prostate cancer samples than E2F1 (Figure 2, O and P, and Supplemental Figure 4, C and D), suggesting that E2F3 may be the primary E2F member driven by RB1 loss to regulate ACSL4 expression in prostate cancer. This evidence concerns the gene RB1 and Familial prostate cancer.