1 mM UA treatment aggravated 0.5 mM ISO induced moderate heart failure in Tg(fli1:EGFP) zebrafish larvae at 72, 96 and 120 hpf (Fig. 4a), quantified by around 100 percent increased cardiovascular cavity size (B), decreased heart beats per minute (C) and up to 20-fold increased nppb expression (D). Here, NPPB is linked to heart failure.