Finally, we identified HSF1 as a core DNB gene and found that HSF1 and the DNB gene MAPKAPK2 may regulate the neighbouring gene SERPINE1, with all three potential biomarkers of the pathological preaggregation of α-Syn, and combined with clinical data, we identified MAPKAPK2 in peripheral blood as a potential biomarker for the early PD diagnosis based on pathological pre-aggregation of α-Syn. Here, HSF1 is linked to Parkinson disease.