The western blot analysis data revealed that the p-Rictor (Ser1591), p-Sin1(Thr86), and p-Akt (Ser473) levels were all downregulated in the GDM fetal lungs (p < 0.001) (Fig. 2B), suggesting that the mTORC2 activity is suppressed under GDM. This evidence concerns the gene AKT1 and gestational diabetes.