After confirming the expression of OVA in B16-OVA cell line (Supplemental Figure 4B), antigen-specific CD8+ T cells with normal or diminished RIG-I expression were cocultured with B16-OVA tumor cells in vitro, which showed that Rig-I knockdown significantly elevated the killing efficiency of antigen-specific CD8+ T cells (Supplemental Figure 4C). Here, CD8A is linked to neoplasm.