Klotho is a pleiotropic protein that down-regulates a variety of cytokines and growth factors, such as IGF-1, Wnt/β-catenin and TGF-β1, and plays a variety of biological functions such as regulating cellular senescence, inhibiting apoptosis, inflammation, oxidative stress and regulating calcium and phosphorus metabolism (12, 108, 109), which plays a critical role in delaying renal aging, protecting the kidney from acute and chronic injury, promoting restoration of renal function and delaying the progression of RF and CKD (85, 86, 110). Here, TGFB1 is linked to chronic kidney disease.