For example, alanine mutagenesis of O-GlcNAc sites in the DNA-binding domain of both mouse and human Oct4 (T228A and T235A, respectively) reduces Oct4 transcriptional activity in mouse embryonic fibroblasts and human cancer cells, respectively (Jang et al., 2012; Constable et al., 2017). The gene discussed is POU5F1; the disease is cancer.