To examine the in vivo effects of TNFR2 ablation on the development of MC38 and CT26 colon cancers, we subcutaneously inoculated MC38/TNFR2-/- and control cells (MC38/WT, 500,000 cells) into the right flank of C57BL/6 mice, or CT26/TNFR2-/- and control cells (CT26/WT, 200,000 cells) into the right flank of BALB/c mice, respectively. Here, TNFRSF1B is linked to malignant colon neoplasm.