In order to further define the role of TNFR2 on tumor development, we used CRISPR/Cas9 technology to knock out TNFR2 gene in MC38 and CT26 colon cancer cells (designated as MC38/TNFR2-/- and CT26/TNFR2-/-, respectively). Here, TNFRSF1B is linked to colonic neoplasm.