Furthermore, the simultaneous induction of both apoptotic pathways in U2OS and HOS cells by HO-3867 is through the JNK signal transduction pathway, contributing to a further understanding of the molecular insight accountable for the apoptotic properties of HO-3867 on human osteosarcoma cells to potentially treat osteosarcoma patients. This evidence concerns the gene MAPK8 and osteosarcoma.