Gel+DOX+CD‐Cur could down-regulate Bcl2, which is closely associated with the prognosis of osteosarcoma patients 194, and up-regulate the protein level of caspase 3, indicating that dual drug nanotechnology improves cytotoxicity consequences and promotes the pro-apoptotic effect of DOX and curcumin bioavailability 114. This evidence concerns the gene CASP3 and osteosarcoma.