In human osteosarcoma Saos-2 cells, EF-24 has a higher potency than curcumin to inhibit cell growth and to induce cell death, change nuclear morphology, and activate caspases 3 and 7 through the Fas/PARP axis, indicating that EF-24 strongly induces apoptosis through both the death receptor-mediated extrinsic pathway and the mitochondrial-mediated intrinsic pathway 158. Here, CASP3 is linked to osteosarcoma.