Additionally, culturing RAW246.7 cells with HCC-conditioned medium from METTL3/YTHDF1-KD HCC cells revealed significantly decreased expression of osteoclast differentiation/activation markers including RNAKL, C-fos, Acp5, Ctsk, Nfat-c1, and Dc-stamp (Fig. 1G and Supplementary Fig. 1E). The gene discussed is CTSK; the disease is hepatocellular carcinoma.