Moreover, using a permanent mouse model of acute MI and ex vivo isolated primary cardiac fibroblasts treated with rCTHRC1 protein, we observed the reduced expression of downstream proteins involved in non-canonical WNT5A-PCP signaling pathway in C1KO mice, whereas the levels of these proteins were up-regulated in the isolated primary cardiac fibroblasts from WT mice, which was stimulated with rCTHRC1 protein. The gene discussed is WNT5A; the disease is myocardial infarction.