Using multiple gene signatures from clinical trials or those widely used to evaluate the ICB-responsiveness of the tumor microenvironment 28-31, we further revealed that the microenvironment of tumors with chromothripsis was relatively less sensitive to ICB treatment (POPLAR signature, median 1.27 × 10-1 vs 8.77 × 10-2, P = 1.3 × 10-10; Checkmate 275 signature, median 2.15 × 10-1 vs 1.78 × 10-1, P = 5.6 × 10-14; IFN-γ-related signature, median 2.83 × 10-1 vs 2.56 × 10-1, P = 4.9 × 10-14; IMvigor210 signature, median 7.30 × 10-2 vs 2.28 × 10-2, P < 2.2 × 10-16; Figure 1F). Here, IFNG is linked to neoplasm.