Consistently, we next measured the expression of ZSCAN1 in BC and normal cell lines and both western blot and qRT-PCR showed the decreased ZSCAN1 expression in BC cell lines, including basal (MDA468), luminal (MCF7, T47D, and BT474), and claudin-low (MDA231) cells (33), versus HMLE normal cell line (34) (Figure 1E, P < 0.01 for western in all cells; Figure 1F, P < 0.001 for qRT-PCR in all cells), indicating the tumor suppressor role of ZSCAN1 in BC. This evidence concerns the gene ZSCAN1 and breast cancer.