We further discovered that JAG1+ cells were mainly distributed in epithelial cells, endothelial cells, fibroblasts, and myeloid cells Signal flows associated with the immunosuppressive microenvironment and tumor angiogenesis are stronger in JAG1-positive samples, which also had significantly activated ligand-receptor interaction in SPP1-(ITGAV+ITGB5), SPP1-(ITGAV+ITGB1), and SPP1-(ITGA8+ITGB1). Here, SPP1 is linked to neoplasm.