In addition, RT-qPCR on the mRNA of five prostate cancer cell lines and one normal prostate cell line showed that KCNJ16 was upregulated in prostate cancer cell lines and was a DEG in patients with prostate cancer who had a Gleason score (GS) = 6 and GS ≥ 7. This evidence concerns the gene KCNJ16 and Familial prostate cancer.