Interestingly, we also detected reductions in disease-associated microglia (DAM)/microglial neurodegenerative disease (MGnD) genes such as Cst7, Clec7a, and Spp1 (Fig. 4l), suggesting that microglia adopted a dampened DAM/MGnD state despite no changes in IBA1+ microglia staining. This evidence concerns the gene SPP1 and neurodegenerative disease.