In the present study, after LDHA was split into several sections, it was found that FOXK2-142aa could directly bind to the △161–218 C-terminal domain of LDHA and phosphorylated LDHA at Tyr10 site, thereby forming the FOXK2-142aa/LDHA signaling pathway to activate the Warburg effect in HCC. The gene discussed is LDHA; the disease is hepatocellular carcinoma.