We found that PRMT1 loss resulted in the loss of SMARCA4 binding at TSSs globally, and the loss of SMARCA4 binding at gene regions of TNS4 and EGFR in both SMARCA4-WT and SMARCA4-R1157W mutant cells, suggesting that H4R3me2a is critical for SMARCA4 binding to chromatin of CRC cells (Fig. 3d; Supplementary Fig. 4d). The gene discussed is SMARCA4; the disease is colorectal carcinoma.