Moreover, as six cases of R1157W mutation patients were all heterozygous (Fig. 1b), we have constructed the SMARCA4-R1157W heterozygous (HE) and homozygous (HO) knock-in mutants using the CRISPR/Cas9 editing system in HCT116 CRC cells. Here, SMARCA4 is linked to colorectal carcinoma.