To map tissue-specific neutrophils during gastric tumorigenesis, we performed fluorescence-activated cell sorting (FACS) to isolate CD45+CD11b+Ly6G+ cells from bone marrow (BM), spleen (SP), peripheral blood (PB), and GC tissues of mice (n = 3) bearing subcutaneous GC, and performed scRNA-Seq analysis on these cells (Fig. 1A). Here, ITGAM is linked to gastric cancer.