The expression of most PRGs, including CASP3, CASP4, CASP6, CASP8, CASP9, GPX4, GSDMA, GSDMB, GSDMC, GSDMD, GSDME, NLRP1, NLRP7, NOD1, NOD2, PJVK, PRKACA, PYCARD, PLCG1, SCAF11 and TIRAP, was significantly increased in HCC tissues compared with normal tissues (Figure 1A, 1B). This evidence concerns the gene GSDME and hepatocellular carcinoma.