To determine whether ITA/Oxgr1 signaling participates in airway pathogen clearance, WT, Oxgr1–/–, and Irg1–/– mice were subjected to intranasal infection with the P. aeruginosa strain PAO1, and BAL and lung tissue were collected 12 hours after infection to count CFU (Figure 5A). This evidence concerns the gene OXGR1 and infection.