To assess whether the decrease in cell viability following LGG-SN treatment was associated with a concurrent increase in cell death, total protein samples from cancer cells treated for 48 h either with increasing concentration of LGG-SN or CTRL complete medium, or puromycin as positive control, were tested for the expression of cleaved Caspase-3 (c-Caspase-3), an effector of apoptosis. This evidence concerns the gene CASP3 and cancer.