As in the stratum radiatum, we observed an increased prevalence of direct contacts between astrocytes and dendritic spines in the APP-PS1 mice compared to C57BL/6J control mice (Control 1.342 ± 0.2394 contacts per astrocyte vs AD 2.529 ± 0.3829 contacts per astrocyte, p = 0.0055) (Fig. 3D). Here, APP is linked to Alzheimer disease.