KEGG analysis revealed the following metabolic pathways with a significant difference: Rheumatoid arthritis, Fluid shear stress and atherosclerosis, Type I diabetes mellitus, Leishmaniasis, MAPK signaling pathway, Th1 and Th2 cell differentiation, IL-17 signaling pathway, Hematopoietic cell lineage, Chagas disease (American trypanosomiasis), Th17 cell differentiation, TNF signaling pathway, and Yersinia infection. The gene discussed is TNF; the disease is American trypanosomiasis.