After 10 weeks of HFD feeding (HF235; 4655 kcal/kg; 37.5% carbohydrate, 27.5% fat, 17% protein), WT mice given 43 μg per day of rcREG3A for 28 days showed improvement in metabolic syndrome and associated traits (changes in lipid profile, leptin, glucose tolerance) not seen in mice given the vehicle (Fig. 5). This evidence concerns the gene LEP and metabolic syndrome.