GAPDH and thyroid cancer: To elucidate the molecular mechanism underlying PAC-dependent induction of apoptosis in thyroid cancer cells, CAL-62 cells were either sham-treated (DMSO) or challenged with PAC (10 μM) for 24, 48 and 72 h, and then cell lysates were prepared and protein levels were assessed by immunoblotting using specific antibodies, while GAPDH was used as internal control.